Via overexpression in the yeast Pichia pastoris to enhanced enantioselectivity: A new chapter in the application of pig liver esterase
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P-65: Effective Parameters on the Bovine Follicle Stimulating Hormone Expression in The Pichia Pastoris System
Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...
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Phytic acid is the dominant form of phosphorous in plant seeds. However, phytic acid cannot beutilized by animals and causes them serious phosphate deficiency. Utilization of phytase as ananimal feed additive can affect liberation of phosphor and its mineral availability. In this study,heterologous expression of the A. niger phyA gene was investigated in the methylotrophic yeastsP. pastoris and...
متن کاملExpression of the VP2 gene of classical D78 infectious bursal disease virus in the methylotrophic yeast Pichia pastoris as a secretory protein
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector...
متن کاملEnhancement of Alpha 1-antitrypsin Production in Pichia pastoris by Designing and Optimizing Medium Using Elemental Analysis
Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the potent inhibitor of a whole range of serine proteases and protects tissues against their destructive effects. The human plasma-derived AAT, which is currently used to augment the AAT level in patients, is limited due to high cost and source limitation. Recombinant production of AAT can be considered as a ...
متن کاملP-205: Production of Recombinant Fish FSH Hormone in Pichia Pastoris
Background: Follicle-stimulating hormone (FSH) belongs to the family of glycoprotein hormones that composing alpha and beta subunits with non-covalently bonds. This hormone involve in regulation of the reproductive processes such as gamete generation and follicular growth. Injection of the hormone in most of fish species increases 17 beta-estradiol production by ovarian tissue and also stimulat...
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